KMID : 0578320190420080589
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Molecules and Cells 2019 Volume.42 No. 8 p.589 ~ p.596
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Loss of ¥âPix Causes Defects in Early Embryonic Development, and Cell Spreading and PlateletDerived Growth Factor-Induced Chemotaxis in Mouse Embryonic Fibroblasts
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Kang Tae-In
Lee Seung-Joon Kwon Young-Hee Park Dong-Eun
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Abstract
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¥âPix is a guanine nucleotide exchange factor for the Rho family small GTPases, Rac1 and Cdc42. It is known to regulate focal adhesion dynamics and cell migration. However, the in vivo role of ¥âPix is currently not well understood. Here, we report the production and characterization of ¥âPix-KO mice. Loss of ¥âPix results in embryonic lethality accompanied by abnormal developmental features, such as incomplete neural tube closure, impaired axial rotation, and failure of allantoischorion fusion. We also generated ¥âPix-KO mouse embryonic fibroblasts (MEFs) to examine ¥âPix function in mouse fibroblasts. ¥âPix-KO MEFs exhibit decreased Rac1 activity, and defects in cell spreading and platelet-derived growth factor (PDGF)-induced ruffle formation and chemotaxis. The average size of focal adhesions is increased in ¥âPix-KO MEFs. Interestingly, ¥âPix-KO MEFs showed increased motility in random migration and rapid wound healing with elevated levels of MLC2 phosphorylation. Taken together, our data demonstrate that ¥âPix plays essential roles in early embryonic development, cell spreading, and cell migration in fibroblasts.
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KEYWORD
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¥âPix, cell motility, embryonic lethality, focal adhesion, mouse embryonic fibroblast
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