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KMID : 0578320200430070519
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Molecules and Cells
2020 Volume.43 No. 7 p.519 ~ p.599
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Mapping Cellular Coordinates through Advances in Spatial Transcriptomics Technology
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Teves Joji Marie
Won Kyoung-Jae
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Abstract
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Complex cell-to-cell communication underlies the basic processes essential for homeostasis in the given tissue architecture. Obtaining quantitative gene-expression of cells in their native context has significantly advanced through single-cell RNA sequencing technologies along with mechanical and enzymatic tissue manipulation. This approach, however, is largely reliant on the physical dissociation of individual cells from the tissue, thus, resulting in a library with unaccounted positional information. To overcome this, positional information can be obtained by integrating imaging and positional barcoding. Collectively, spatial transcriptomics strategies provide tissue architecture-dependent as well as position-dependent cellular functions. This review discusses the current technologies for spatial transcriptomics ranging from the methods combining mechanical dissociation and single-cell RNA sequencing to computational spatial re-mapping.
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KEYWORD
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cellular communication, single-cell RNA, spatial transcriptomics, tissue architecture
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