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KMID : 0607119950020010099
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Inha Medical Journal
1995 Volume.2 No. 1 p.99 ~ p.112
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Microvascular Changes by Increased leukocytes during Cerebral Ischemia-Reperfusion
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Abstract
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We made an in vivo model of cerbral ischemia-reperfusion and can obtain rhodamine 6G(0.5mg/kg i.v. loading dose, 0.5 mg/kg/h i.v. maintenance dose)-labelled leukocytes flowing within pial venules of aneshetized piglets. A closed cranial window
was
implanted over the parietal cortex, and hydrogen clearance apparatus and laser doppler fowmetry were applied on bifrontal and left parietal areas for cerebral blood flow. We selected pial venules of 50-100㎛diameter with fluorescence microscope
(Olympus
BHM). We found there are the photodamage and chemical damage in the control group and we though that the ideal model is the short exposed duration of light source with rhodamine 6G. Few labelled leuckocytes were observed adhering to the
endothelium
of
venules in preischemic state. But after ischenia-reperfusion injury was made by reversible subclavian and brachiocephalic occlusion, a dramatic increase in adherent leukocytes and no-reflow were observed at 30, 60, and 90 min. cortical blood flow
was
markedly decreased by 25-40 %(p<0.05%). These findings suggest that the movement of leukocytes may be the key event mediating the no-reflow, which may contribute to hypoperfusion following global cerebral ischemia.
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KEYWORD
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