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KMID : 0608520100160030155
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Korean Journal of Oriental Medicine
2010 Volume.16 No. 3 p.155 ~ p.166
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Experimental Study about Pathway of Aconiti Ciliare Tuber on Allergic Reaction of Inflammation
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Kim Won-Ill
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Abstract
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Objective: The purpose of this study was to examine the pathway of anti-allergic effects of Aconiti Ciliare Tuber (ACT).
Methods: We examined cell viability, β-hexosaminidase release, pro-inflammatory cytokines secretion and mRNA expressions, nuclear factor-kappa B (NF-κB) (p65) activation, inhibitor kappa B-alpha (IκB-α) degradation, and MAPKs activation from RBL-2H3 cells pre-treatment by ACT of 1.0 ㎎/㎖, 2.0 ㎎/㎖ separately.
Results: We observed that ACT reduced the secretion of β-hexosaminidase, TNF-α, IL-4 and the expression of COX-2 mRNA in RBL-2H3 cells. Futhermore, ACT inhibited the levels of activation of NF-κB (p65) protein, ERK MAPK, and degradation of IκB-α in RBL-2H3 cells.
Conclusions: These results show that ACT has an anti-histamine effect and inhibitory effect of NF-κB (p65) through regulation of IκB-α degradation. This improves that ACT could be used as an anti-allergic medicine.
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KEYWORD
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Aconiti Ciliare Tuber, β-hexosaminidase, TNF-α, IL-4
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