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KMID : 0613820050150060923
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Journal of Life Science
2005 Volume.15 No. 6 p.923 ~ p.927
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Development of Bactericidal Yeast Strain by Expressing the Leucocin A Gene on the Cell Surface of Saccharomyces cerevisiae
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Lee Sang-Hyun
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Abstract
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In order to develop yeast cells that produce a bacteriocin on their cell surfaces, the 114 bp Leucocin A gene with stop codon was ligated into pYD1, an yeast vector. The recombinant DNA, pYD1-LeucoA was used to transform yeast (Saccharomyces cerevisiae) cells. Yeast cells harboring pYD1-LeucoA showed antibacterial activity against Bacillus subtilis. To confirm these bacteriocidal yeast cells possess the Leucocin A gene, PCR was performed with plasmid prepared from transformed yeast cells as a template and two Leucocin A-specific primers. In this study, bacteriocidal yeast cells that can be used as an antibiotic or a food preservative were developed
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