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KMID : 0613820090190060799
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Journal of Life Science
2009 Volume.19 No. 6 p.799 ~ p.803
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Evaluation of Radiation-induced Apoptosis in Seminiferous Tubule of ICR Mouse after Gamma Irradiation
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Jang Jong-Sik
Kim Joong-Sun
Kim Jong-Choon
Kim Seong-Ho
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Abstract
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The killing of male germ cells by radiation and other toxicants has recently been attributed to apoptosis, but a critical evaluation of the presence of the different features of apoptosis in each epithelial stage has not been performed. In this study, mouse testes exposed to radiation were examined by light microscopy and terminal transferase-mediated end labeling (TUNEL) with periodic acid-Schiff (PAS) stains to determine whether the cells were apoptotic according to several criteria. Apoptosis was easily recognized by the presence of peroxidase-stained, entirely apoptotic bodies. In the TUNEL-positive cells or bodies, the stained products correlated precisely with the typical morphologic characteristics of apoptosis as seen at the light microscopic level. The changes that occurred from 0 to 24 hours after exposing the mice to 2 Gy of gamma-rays (2 Gy/min) were examined. The numbers of apoptotic cells reached a peak at 12 hours after irradiation and then declined. The mice that received 0-8 Gy of gamma-rays were examined 8 hours after irradiation. Dose-response relationships were generated for each stage of the epithelial cycle by counting TUNEL-positive cells. The dose-response curves were linear-quadratic [y=(-0.014±0.009)D²+(0.31±0.697)D+0.3575. Where y=the number of apoptotic cells per seminiferous tubule, and D=the irradiation dose in Gy, r²=0.9] and there was a significant relationship between the frequency of apoptotic cells and the radiation dose. Although the maximum response was produced by 8 Gy, even 0.5 Gy induced marked changes. These changes were most pronounced in B spermatogonia of stage Ⅴ and the spermatocyte at the mitotic cells of stage ?.
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KEYWORD
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Gamma-ray, apoptosis, seminiferous tubule, epithelial stage
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