KMID : 0616120170480010093
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Korean Journal of Pharmacognosy 2017 Volume.48 No. 1 p.93 ~ p.96
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Simultaneous Determination of (+)-Pseudoephedrine and (-)-Ephedrine in Ephedra intermedia by HPLC-UV
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Jeong Bi-Rang
Yoon Yoo-Sik Shin Soon-Shik Kwon Yong-Soo Yang Hee-Jung
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Abstract
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Ephedra alkaloids, (-)-ephedrine, (+)-pseudoephedrine, (-)-N-methylephedrine, (+)-N-methylpseudoephedrine, (-)- norephedrine and (+)-norpseudoephedrine, from ephedra herb are sympathomimetic agonists causing an increase of metabolism, blood pressure and perspiration. In this study, we developed the validation method of (+)-pseudoephedrine and (-)-ephedrine, two major ephedra alkaloids in Ephedra spp., by high-performance liquid chromatography-ultraviolet spectrometer (HPLC-UV). HPLC analysis was performed using a HECTOR-M C18 column operating at 35 ¡É, and UV detection at 215nm. The mobile phase used a gradient flow with 25 mM SDS in water (A) and acetonitrile (B).
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KEYWORD
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HPLC analysis, Ephedra intermedia, Ephedrine, Pseudoephedrine, Method validation
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