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KMID : 0620720030090040249
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Natural Product Sciences
2003 Volume.9 No. 4 p.249 ~ p.255
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Crude Extract of Zizyphi Jujube Semen Protects Kainic Acid-induced Excitotoxicity in Cultured Rat Neuronal Cells
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Park, Jeong Hee
Ban, Ju Yeon/Joo, Hyun Soo/Song, Kyung Sik/Bae, Ki Whan/Seong, Yeon Hee
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Abstract
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Zizypus is one of the herbs widely used Korea and China due to the CNS calming effect. The present study aims to investigate the effect of the methanol extract of Zujube Semen (ZIS) on kainic acid (KA)-induced neurotoxicity in cultured rat cerebellar granule neuron. ZJS, over a concentration range of 0.05 to 5 5 μg/ml, inhibited KA (500 μM)-induced neuronal cell death, which was measured by a trypan blue exclusion test and a 3-[4,5-dimethylthiazol-2-y1]-2,5-dipheny1-tetrazolium bromide (MTT) assay. Pretreatment of ZJS (0.5 μg/ml) inhibited KA (50 μM)-induced elevation of cytosolic calcium concentration ([Ca^(2+)]_(c), which was measured by a fluorescent dye, Fura 2-AM, and generation of reactive oxygen species (ROS). ZJS (0.5μg/ml) inhibited glutamate release into medium induced by KA (500 μM), which was measured by HPLC. These results suggest that ZJS prevents KA-induced neuronal cell damage in vitro.
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