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KMID : 0624219990070000035
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Focus on Genetic Science
1999 Volume.7 No. 0 p.35 ~ p.49
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Differential role of Saccahromyces cerevisiae Ste5 G1 cell cycle arrest and mating
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Choi, You-Jeong
Kim, Sun-Hong/Park, Ki-Sook/Choi, Kang-Yell
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Abstract
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Saccharomyces cerevisiae Ste5 is a scaffold protein which recruits many pheromone signaling molecules to sequester the pheromone pathway from other homologous MAPK (mitogen-activated protein kinase) pathwasys. G1 cell cycle arrest and mating are two different physiological consequences of pheromone signal transduction and Ste5 is required for both processes. However, the roles of Ste5 in G1 arrest and mating are not fully understood. To understand the roles of Ste5 better, we isolated 150 G1 cell cycle arrest defective STE5 mutants by chemical mutagenesis of the gene. Here, we found that two G1 cell cycle arrest defective STE5 mutants (ste5M^(D248V) and ste5^(△-776)) retained mating capacity. When overproduced in a wild type strain, several ste5 mutants also showed different dominant phenotypes for G1 arrest and mating. Isolation and characterization of the mutants suggested separable roles of Ste5 in G1 arrest and mating of S. cerevisiae. In addition, the roles of Asp-248 and Tyr-421, which are important for pherome signal transduction were further characterized by site-directed mutagenesis studies.
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KEYWORD
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