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KMID : 0624620080410040316
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BMB Reports
2008 Volume.41 No. 4 p.316 ~ p.321
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Regulation of macrophage inflammatory protein-2 gene expression in response to 2,4-dinitrofluorobenzene in RAW 264.7 cells
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Kim Dong-Bum
Kim Jin-Ho
Kwon Sang-Hoon
Kim Young-Jin
Lee Soo-Hyoung
Lee Young-Hee
Seo Jae-Nam
Park Cheung-Seog
Park Kui-Lea
Kwon Hyung-Joo
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Abstract
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Several skin sensitizers, like 2,4-dinitrofluorobenzene (DNFB), are known to provoke contact hypersensitivity responses after topical application. Here, we show that DNFB can upregulate macrophage inflammatory protein-2 (MIP-2) expression in RAW 264.7 cells via a mechanism that is largely dependent on mitogen-activated protein kinase (MAPK) signaling pathways. ELISA-based transcription factor activation assays and chromatin immunoprecipitation assays revealed that functional interaction between AP-1 and MIP-2 promoter element is necessary for MIP-2 gene expression by DNFB. Interestingly, topical application of DNFB to NC/Nga mice increased MIP-2 expression in dermis, suggesting that MIP-2 contributes to the leukocyte infiltration associated with atopic dermatitis. These results provide additional insight of the mechanism of contact hypersensitivity induced by contact sensitizers. [BMB reports 2008; 41(4): 316-321]
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KEYWORD
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AP-1, DNFB, Macrophages, MIP-2
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