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KMID : 0624620080410070511
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BMB Reports
2008 Volume.41 No. 7 p.511 ~ p.515
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Examination of specific binding activity of aptamer RNAs to the HIV-NC by using a cell-based in vivo assay for protein-RNA interaction
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Jeong Yu-Young
Kim Seon-Hee
Jang Soo-In
You Ji-Chang
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Abstract
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The nucleocapsid (NC) protein of the Human Immunodeficiency Virus-1 plays a key role in viral genomic packaging by specifically recognizing the Psi(Ψ) RNA sequence within the HIV-1 genome RNA. Recently, a novel cell-based assay was developed to probe the specific interactions in vivo between the NC and Ψ-RNA using E.coli cells (J. Virol. 81: 6151-55, 2007). In order to examine the extendibility of this cell-based assay to RNAs other than Ψ-RNA, this study tested the RNA aptamers isolated in vitro using the SELEX method, but whose specific binding ability to NC in a living cellular environment has not been established. The results demonstrate for the first time that each of those aptamer RNAs can bind specifically to NC in a NC zinc finger motif dependent manner within the cell. This confirms that the cell-based assay developed for NC-Ψ interaction can be further extended and applied to NC-binding RNAs other than Ψ-RNA. [BMB reports 2008; 41(7): 511-515]
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KEYWORD
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Cell-based assay, HIV nucleocapsid protein, Protein-RNA interaction, Psi (Ψ), RNA aptamer
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