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KMID : 0624620110440070484
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BMB Reports
2011 Volume.44 No. 7 p.484 ~ p.489
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Transduced Tat-Annexin protein suppresses inflammationassociated gene expression in lipopolysaccharide (LPS)-stimulated Raw 264.7 cells
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Lee Sun-Hwa
Kim Dae-Won
Back Su-Sun
Hwang Hyun-Sook
Park Eun-Young
Kang Tae-Cheon
Kwon Oh-Shin
Park Jong-Hoon
Cho Sung-Woo
Han Kyu-Hyung
Park Jin-Seu
Eum Won-Sik
Choi Soo-Young
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Abstract
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Annexin-1 (ANX1) is an anti-inflammatory protein as well as an important modulator in inflammation. However, the precise action of ANX1 remains unclear. To elucidate the protective effects of ANX1 on lipopolysaccharide (LPS)-induced murine macrophage Raw 264.7 cells, we constructed a cell-permeable Tat-ANX1 protein. The transduced Tat-ANX1 protein markedly inhibited the expression of cyclooxygenase-2, production of prostaglandin E(2), and generation of pro-inflammatory cytokines in the cells. Furthermore, transduced Tat-ANX1 protein caused a significant reduction in the activation of nuclear factor- kappa B (NF-kB) and mitogen-activated protein kinase (MAPK). The results indicate that Tat-ANX1 inhibits the production of inflammatory response cytokines and enzymes by blocking NF-kB and MAPK. Therefore, Tat-ANX1 protein may be useful as a therapeutic agent against various inflammatory diseases.
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KEYWORD
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Cytokine, Inflammation, MAPK, Protein therapy, Tat- ANX1
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