KMID : 0624620120450010051
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BMB Reports 2012 Volume.45 No. 1 p.51 ~ p.56
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Glucocorticoid treatment independently affects expansion and transdifferentiation of porcine neonatal pancreas cell clusters
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Kim Ji-Won
Sun Cheng-Lin Jeon Sung-Yoon You Young-Hye Shin Ju-Young Lee Seung-Hwan Cho Jae-Hyoung Park Chung-Gyu Yoon Kun-Ho
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Abstract
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The purpose of this study was to determine the effects of duration and timing of glucocorticoid treatment on the expansion and differentiation of porcine neonatal pancreas cell clusters (NPCCs) into ¥â-cells. After transplantation of NPCCs, the ductal cyst area and ¥â-cell mass in the grafts both showed positive and negative correlations with duration of dexamethasone (Dx) treatment. Pdx-1 and HNF-3¥â gene expression was significantly downregulated following Dx treatment, whereas PGC-1¥á expression increased. Pancreatic duct cell apoptosis significantly increased following Dx treatment, whereas proliferation did not change. Altogether, transdifferentiation of porcine NPCCs into ¥â-cells was influenced by the duration of Dx treatment, which might have been due to the suppression of key pancreatic transcription factors. PGC-1¥á plays an important role in the expansion and transdifferentiation of porcine NPCCs, and the initial 2 weeks following transplantation of porcine NPCCs is a critical period in determining the final ¥â-cell mass in grafts.
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KEYWORD
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Dexamethasone (Dx), Diabetes mellitus, Neonatal porcine pancreas cell clusters (NPCCs), Pancreatic ¥â-cells, Transdifferentiation
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