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KMID : 0624620150480030153
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BMB Reports
2015 Volume.48 No. 3 p.153 ~ p.158
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The effects of PEP-1-FK506BP on dry eye disease in a rat model
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Kim Dae-Won
Lee Sung-Ho
Ku Sae-Kwang
Lee Ji-Eun
Cha Hyun-Ju
Youn Jong-Kyu
Kwon Hyeok-Yil
Park Jong-Hoon
Park Eun-Young
Cho Sung-Woo
Han Kyu-Hyung
Park Jin-Seu
Eum Won-Sik
Choi Soo-Young
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Abstract
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As FK506 binding proteins (FK506BPs) are known to play an important role in the regulation of a variety of biological processes related to cell survival, this study was designed to examined the protective effects of FK506 binding protein 12 (FK506BP) on low humidity air flow induced dry eye in a rat model using transduced PEP-1-FK506BP. After the topical application of PEP-1-FK506BP, tear volumes were markedly increased and significant prevention of cornea damage was observed compared with dry eye rats. Further, immunohistochemical analysis demonstrated that PEP-1-FK506BP markedly prevented damage to the cornea, the bulbar conjunctiva, and the palpebral conjunctiva epithelial lining compared with dry eye rats. In addition, caspase-3 and PARP expression levels were found to be decreased. These results demonstrated that topical application of PEP-1-FK506BP significantly ameliorates dry eye injury in an animal model. Thus, we suggest that PEP-1-FK506BP can be developed as a new ophthalmic drop to treat dry eye diseases.
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KEYWORD
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Dry eye disease, FK506BP, Tear volume, Protein therapy, Protein transduction
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