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KMID : 0624620150480070413
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BMB Reports
2015 Volume.48 No. 7 p.413 ~ p.418
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DEPDC1 is a novel cell cycle related gene that regulates mitotic progression
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Mi Yan
Zhang Chundong
Bu Youquan
Zhang Ying
He Longxia
Li Hongxia
Zhu Huifang
Li Yi
Lei Yunlong
Zhu Jiang
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Abstract
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DEPDC1 is a recently identified novel tumor-related gene that is upregulated in several types of cancer and contributes to tumorigenesis. In this study, we have investigated the expression pattern and functional implications of DEPDC1 during cell cycle progression. Expression studies using synchronized cells demonstrated that DEPDC1 is highly expressed in the mitotic phase of the cell cycle. Immunofluorescence assays showed that DEPDC1 is predominantly localized in the nucleus during interphase and is redistributed into the whole cell upon nuclear membrane breakdown in metaphase. Subsequently, siRNA-mediated knockdown of DEPDC1 caused a significant mitotic arrest. Moreover, knockdown of DEPDC1 resulted in remarkable mitotic defects such as abnormal multiple nuclei and multipolar spindle structures accompanied by the upregulation of the A20 gene as well as several cell cycle-related genes such as CCNB1 and CCNB2. Taken together, our current observations strongly suggest that this novel cancerous gene, DEPDC1, plays a pivotal role in the regulation of proper mitotic progression.
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KEYWORD
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Cancer, Cell cycle, DEPDC1, Mitosis, Nuclear protein
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