|
KMID : 0644120070200010050
|
|
Journal of Oriental Gynecology
2007 Volume.20 No. 1 p.50 ~ p.60
|
|
|
Anti-proliferation effect of Gyulyupsanbyonbang extracts on MCF-7 cells
|
|
Yang Seung-Jung
Jo Sung-Hee
Cho Su-In
Jo Hyun-Jung
|
|
|
Abstract
|
|
|
Purpose:This investigation was undertaken to evaluate the anti-proliferation, in hexane, chloroform, ethyl acetate, butanol and water fraction from extract of Gyulyupsanbyonbang(GYSB) using MCF-7 human breast cancer cells.
Methods: GYSB was added to distilled water(1500ml) and was boiled then filtered. The residue was suspended in distilled water and extracted with hexane, chloroform, ethyl acetate, butanol and water. MCF-7 cells were cultured in RPMI1640 complex badge, NIH3T3 was cultered in 37$^{\circ}$C, 5% moisture incubator of carbon dioxide with Dulbecco’s Modified Eagle Medium(DMEM) supplemented with 10% fetal bovine serum and antibiotics. Cell cytotoxicity test about cancer cell was measured used MTT assay.
Results:When it synthesizes a result, hexane and butanol fraction had shown anti-proliferation effect and safety together, and those anti-proliferation effect operating selectively appeared. Ethyl acetate fraction had anti-proliferation effect however, it was not selective. The Chloroform and water soluble fraction did not almost appear anti-proliferation effect.
Conclusion:I can conclude that GYSB have anti-proliferation effect and safety together on MCF-7 cells. It suggest that GYSB may be useful for brest cancer patients.
|
|
|
KEYWORD
|
|
|
human breast cancer, MCF-7, Gyulyupsanbyonbang, anti-cancer, anti-proliferation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|