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KMID : 0644120070200030035
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Journal of Oriental Gynecology
2007 Volume.20 No. 3 p.35 ~ p.44
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Anti-proliferative Effect of Ulmi Pumilae Cortex Extracts on MCF-7 cells
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Jo Sung-Hee
Cho Su-In
Na Won-Min
Yang Seung-Jung
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Abstract
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Purpose: This study was conducted to investigate the anti-proliferative effects of Ulmi Pumilae Cortex Extracts(UPCE) on MCF-7(human, breast, adenocaecinoma) and NIH3T3 (human, murine, fibroblast).
Methods:MCF-7 cells and NIH3T3 cells were cultured and seeded in cell culture plates, respectively. UPC was extracted with hot water and then further fractionated it into five types: hexane, chloroform, ethyl acetate, butanol, and water soluable fractions. These five different fractions from UPCE were tested for their anti-proliferative effects on MCF-7 cells and NIH3T3 cells by MMT assay.
Results:Among the five solvent-fractions of UPCE, n-hexane fraction and ethyl acetate fraction showed a strong anti-proliferative effects on MCF-7 cells but they displayed significant cytotoxicity on NIH3T3 cells, too. On the other hand, chloroform fraction showed a marked anti-proliferative effects on MCF-7 cells and low cytotoxicity on NIH3T3 cells.
Conclusion: Chloloform fraction from UPCE showed selective anti-cancer activities on human breast cancer cell MCF-7 relatively to the other fractions.
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KEYWORD
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Ulmi Pumilae Cortex, MCF-7, human breast cancer, anti-proliferative effects
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