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KMID : 0665220180310060957
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Korean Journal of Food and Nutrition
2018 Volume.31 No. 6 p.957 ~ p.963
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Quantitative Analysis of Tangshenoside Ⅰ and Lobetyolin from Korean Deoduk (Codonopsis lanceolata)
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Hwang Byung-Soon
Kim Ji-Young
Jang Mi
Kim Gi-Chang
Park Young-Hee
Hwang In-Guk
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Abstract
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Deoduk (Codonopsis lanceolata) has a complex chemical composition that includes polyphenols, saponins, amino acids, and other unidentified compounds. The contents of tangshenoside and lobetyolin are considered as standard of quality evaluation of Deoduk. In this study, an ultra-performance liquid chromatography (UPLC) method was developed for the quantitative determination of the two marker constituents, tangshenoside and lobetyolin. The methods for determining the standards of quality were validated by measuring their linearity, specificity, limit of detection (LOD), limit of quantification (LOQ), precision, and accuracy using UPLC. Reversed-phase UPLC analysis was conducted quantitatively to identify individual tangshenoside and lobetyolin in Deoduk extracted with 50% (v/v) aqueous ethanol. We used 21 samples to carry out quantitative analysis of tangshenoside and lobetyolin. Based on their dry weights, the levels of tangshenoside and lobetyolin were 0.36~3.54 mg/g, 0.24~1.29 mg/g, respectively. These results will be valuable as basic data for standardization of Korean Deoduk.
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KEYWORD
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Codonopsis lanceolata, tangshenoside, lobetyolin, UPLC
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