KMID : 0665420200350030278
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Korean Journal of Food Culture 2020 Volume.35 No. 3 p.278 ~ p.284
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Monitoring of Biotin Content in Frequently Consumed Foods in Korea
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Kwon Ji-Hyun
Cheon Won-Young Lee Sang-Hoon Choi Yong-Min Kim Young-Hwa
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Abstract
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In this study, biotin (vitamin B7) contents of frequently consumed foods in Korea were determined by usingimmunoaffinity column in conjunction with high-performance liquid chromatography (HPLC). The biotin contents of 24foods of plant origin and 27 foods of animal origin were selected. The highest biotin contents in frequently consumed foodsof plant origin were found in red beans (Huinguseul; 11.475 ¥ìg/100 g). On the other hand, biotin was not detected in anyvarieties of sorghum. For frequently consumed foods of animal origin, salted pollack roe (7.486 ¥ìg/100 g) showed thehighest biotin content. However, beef and fish contained less biotin. All biotin analyses were conducted under analyticalquality control. The limits of detection and limits of quantification of biotin were 0.007 and 0.023 ¥ìg/100 g, respectively, andthe accuracy/recovery percentage was 95.35-105.02%. The precision values were 4.041% (repeatability) and 3.835%(reproducibility). Taken together, our data provide reliable data on the biotin contents of frequently consumed foods inKorea.
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KEYWORD
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Biotin, frequently consumed foods, immunoaffinity column, high-performance liquid chromatography
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