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KMID : 0811719970010010091
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Korean Journal of Physiology & Pharmacology
1997 Volume.1 No. 1 p.91 ~ p.96
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Lipocortin 1 Mediates the Suppressive Effects of Dexamethasone on ConA-induced Proliferative Response and Nitric Oxide Production in Rat Splenic Leukocytes
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Yeon Jin Jang
Hyoungsup Park/Soon A Kang/Sue Jung Yang/Doe Sun Na
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Abstract
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Lipocortin 1 has been proposed as a putative mediator of anti-inflammatory actions of glucocorticoids. We investigated the role of lipocortin 1 in the effect of dexamethasone using rat splenic leukocytes. Concanavalin A(ConA; 1 μg/ml) increased the leukocyte proliferation and nitric oxide(NO) generation, which were measured as [3H]?thymidine uptake by the cells and nitrite accumulation in the culture media, respectively. Dexamethasone suppressed ConA-induced cell proliferation, in a concentration-dependent manner with EC50 around 50nM. The addition of anti-lipocortin l(Anti-LCl) reversed dexamethasone effects: 0.24, 1.2, 6 μg/ml of Anti-LC1 reversed dexamethasone(50 nM)-induced suppression of thymidine uptake by 9±3, 16±3, 36±5, respectively; 0.24, 1.2, and 6 μg/ml of Anti-LCI reversed dexa-methasone-induced decrease of nitrite concentration by 49±16, 61±20, 77±19, respectively. The present data indicate that lipocortin 1 mediates, at least in part, glucocorticoids-induced suppression of leukocyte proliferation and blockade of NO generation.
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KEYWORD
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Lipocortin 1, Dexamethasone, Thymidine uptake, Nitric oxide production,
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