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KMID : 0811720090130030139
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Korean Journal of Physiology & Pharmacology
2009 Volume.13 No. 3 p.139 ~ p.145
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Water Extract of Samultang Reduces Apoptotic Cell Death by H2O2-Induced Oxidative Injury in SK-N-MC Cells
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Lee Gyoung-Wan
Kim Min-Sun
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Abstract
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The purpose of this study was to evaluate the effects of the water extract of Samultang (SMT), a Chinese herb, on apoptotic cell death by H2O2-induced oxidative stress in SK-N-MC cells. A nuclear fragmentation was observed via fluorescence imaging 12 h after exposure to 30?M H2O2 and DNA laddering was detected via agarose electrophoresis gel. In addition, increases in sub-G1 phase and cleavage of the PARP protein were observed. However, treatment with SMT for 2 h prior to H2O2 exposure significantly reduced apoptotic cell death induced by incubation with 30?M H2O2 in SK-N-MC cells. Pre-incubation with water extract of SMT for 2 h prevented the H2O2-induced decrease in mitochondrial transmembrane potential. SMT also attenuated the increase in caspase-3 activity and the breakdown of PARP protein caused by H2O2-induced oxidative stress. These results suggest that the water extract of SMT provides inhibition of apoptotic cell death against oxidative injury in SK-N-MC cells.
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KEYWORD
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Samultang, Apoptosis, Oxidative injury, SK-N-MC
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