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KMID : 0811720090130030175
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Korean Journal of Physiology & Pharmacology
2009 Volume.13 No. 3 p.175 ~ p.179
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P2X7 Receptor-mediated Membrane Blebbing in Salivary Epithelial Cells
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Hwang Sung-Min
Koo Na-Youn
Choi Se-Young
Chun Gae-Sig
Kim Joong-Soo
Park Kyung-Pyo
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Abstract
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High concentrations of ATP induce membrane blebbing. However, the underlying mechanism involved in epithelial cells remains unclear. In this study, we investigated the role of the P2X7 receptor (P2X7R) in membrane blebbing using Par C5 cells. We stimulated the cells with 5 mM of ATP for 1∼2 hrs and found the characteristics of membrane blebbing, a hallmark of apoptotic cell death. In addition, 500?M Bz-ATP, a specific P2X7R agonist, induced membrane blebbing. However, 300?M of Ox-ATP, a P2X7R antagonist, inhibited ATP-induced membrane blebbing, suggesting that ATP- induced membrane blebbing is mediated by P2X7R. We found that ATP-induced membrane blebbing was mediated by ROCK I activation and MLC phosphorylation, but not by caspase-3. Five mM of ATP evoked a biphasic [Ca2＋]i response; a transient [Ca2＋]i peak and sustained [Ca2＋]i increase secondary to ATP-stimulated Ca2＋ influx. These results suggest that P2X7R plays a role in membrane blebbing of the salivary gland epithelial cells.
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KEYWORD
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P2X7 receptor, Membrane blebbing, ROCKI, Ca2＋ influx
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