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KMID : 0811720110150050313
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Korean Journal of Physiology & Pharmacology
2011 Volume.15 No. 5 p.313 ~ p.317
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Intracellular Ca2＋ Mobilization and Beta-hexosaminidase Release Are Not Influenced by 60 Hz-electromagnetic Fields (EMF) in RBL 2H3 Cells
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:Hwang Yeon-Hee
:Song Ho-Sun/:Kim Hee-Rae/:Ko Myoung-Soo/:Jeong Jae-Min/:Kim Yong-Ho/:Ryu Jeong-Soo/:Sohn Uy-Dong/:Gimm Yoon-Myoung/:Myung Sung-Ho/:Sim Sang-Soo
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Abstract
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The effects of extremely low frequency electromagnetic fields (EMF) on intracellular Ca2＋ mobilization and cellular function in RBL 2H3 cells were investigated. Exposure to EMF (60 Hz, 0.1 or 1 mT) for 4 or 16 h did not produce any cytotoxic effects in RBL 2H3 cells. Melittin, ionomycin and thapsigargin each dose-dependently increased the intracellular Ca2＋ concentration. The increase of intracellular Ca2＋ induced by these three agents was not affected by exposure to EMF (60 Hz, 1 mT) for 4 or 16 h in RBL 2H3 cells. To investigate the effect of EMF on exocytosis, we measured beta- hexosaminidase release in RBL 2H3 cells. Basal release of beta-hexosaminidase was 12.3±2.3% in RBL 2H3 cells. Exposure to EMF (60 Hz, 0.1 or 1 mT) for 4 or 16 h did not affect the basal or 1?M melittin-induced beta-hexosaminidase release in RBL 2H3 cells. This study suggests that exposure to EMF (60 Hz, 0.1 or 1 mT), which is the limit of occupational exposure, has no influence on intracellular Ca2＋ mobilization and cellular function in RBL 2H3 cells.
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KEYWORD
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EMF, Ca2＋ mobilization, Exocytosis, Beta-hexosaminidase
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