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KMID : 0828520010050030232
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Journal of the Korean Geriatrics Society
2001 Volume.5 No. 3 p.232 ~ p.239
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Effect of Age and Liver Cirrhosis on the Glutathione Concentration and Glutathione Peroxidase Activity in the Plasma, Erythrocytes and Gastric Mucosa of Human
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백광호/Baik GH
김종혁/백일현/한태호/박현주/박상훈/서중산/Kim JH/Baek IH/Hahn TH/Park HJ/Park SH/Suh JS
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Abstract
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Background : The role of aging in damage to DNA have been of increasing in recent years. DNA damage correlated with biochemical and physiologic changes that are characteristic of cellular impairment in aging and disease. Reduction of oxygen in tissue a number of oxygen free radicals which may induce cellular damge and even cell death. Glutathione its function in reductive processes that are essential for the synthesis(and the degradation) of proteins. formation of deoxyribonucleotide precursors of DNA. regulation of enzymes, and protection of the cell against reactive oxygen compounds and free radicals. The aim of this study was, 1a0 to measure the glutathione concentration and glutathione perosicase activity of erythroyte, plasma, human gastric mucosa in elderly and liver cirhosis patient 2) to investigate a role of glutathione mediated cellular defense mechanism against oxidative stress between in liver cirrhosis patient and in elderly.
Methods : We measured glutathione concentration and glutathione peroxidase activity in the plasma, erythrocytes, gastric mucosa of human in 4 group(Group A : 10 patients of liver cirrhosis and portal hypertensive gastrogathy in age 40~55 years, Group B : same number and disease of patients in age over 65 years, Group C : healthy person of age over 65 years, Group D : control). Glutathione concenration of erythrocyte, plasma and human gastric mucosa was measured by spectrothotometer using Bioxytech GSH-400. Glutathione peroxidase activity of plasma was measureded by Paglia & Valentine method using Bioxytech pl.Gpx and of erythrocyte and human gastric mucosa was measured by using Bioxytedh Gpx.340.
Statistical significance of the different group was determined by ANOVA. A P<0.05 was considered significant.
Results : Glutathione concentration of erythrocytes and gastric mucosa was decreased in Group A, B, C compared to group D. plasma concentration of glutathione was decreased in group A, B compared to group C, D. Activity of glutathione peroxidase was not different in any group(ANOVA, P<0.005)
Conclusion : Even though glutathione concentration of erythrocyte and human gastric mucosa was decresed in elderly and in liver cirrhosis patient, our study shows decreased glutathione related defense mechanism against oxidative stress is different in view of plasma concentration of glutathione.
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