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KMID : 0854720010210020223
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Korean Journal of Asthma, Allergy and Clinical Immunology
2001 Volume.21 No. 2 p.223 ~ p.230
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Role of IL-5-activated eosinophils on collagen gel contraction by lung fibroblasts
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Kim Sang-Hoon
Park Heung-Woo
Kim Sang-Heon
Lee Su-Yeon
Jeon Soon-Young
Chung Jae-Won
Chang Yoon-Seok
Lee Byung-Jae
Kim Yoon-Keun
Min Kyung-Up
Kim You-Young
Cho Sang-Heon
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Abstract
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Background：Subepithelial fibrosis plays a major role in the development of irreversible airway obstruction in ast hma. Eosinophils are major effector cells in allergic inflammation, and it has been suggest ed t hat eosinophil-derived mediators such as transforming growt h factor-β (T GF -β) play a role in t he pat hogenesis of airway remodeling.
Objective : The aim of this study was to evaluat e whet her eosinophils activated by IL-5 plays a major role in the collagen gel contraction by lung fibroblasts.
Method : Various cell numbers of lung fibroblasts were cultured in collagen gels to determine the appropriat e numbers of fibroblasts. Purified human peripheral blood eosinophils were activated by IL-5 for 3 days, and T GF -β mRNA expression was evaluated using semiquantitative RT-PCR. The cultured supernat ants with or without TGF -β were added to t he collagen gel media wit h lung fibroblasts, and collagen gel diameter was serially measured to evaluate collagen gel
contraction.
Results : The amount of collagen gel contraction was significantly associated wit h the number of fibroblasts (p< 0.05), and T GF -β significantly contract ed t he collagen gel to contract in a dose-dependent manner (p< 0.05). However, supernatants derived from IL-5-activat ed eosinophils did not contract the collagen gel compared to controls (p> 0.05). Moreover, expression of T GF -β mRNA in eosinophils was t he same before and aft er stimulus of IL-5.
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KEYWORD
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Eosinophil, TGF-β, lung fibroblast, collagen gel contraction,
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