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KMID : 0854720100300030209
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Korean Journal of Asthma, Allergy and Clinical Immunology
2010 Volume.30 No. 3 p.209 ~ p.215
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Measurement Specific IgE against Recombinant 16-kD and 19-kD Buckwheat Allergens for the Diagnosis of Buckwheat Allergy
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Lee Jae-Hyun
Lee Yong-Won
Sin Yu-Seob
Park Hae-Sim
Hong Chein-Soo
Park Jung-Won
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Abstract
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Background: Buckwheat (BW, Fagopyrum esculentum)-specific IgE (sIgE) measurement is crucial to diagnose BW allergy. However, clinical application is difficult due to a high false-positive rate. Previously we identified 16-kD 2S
storage albumin and 19-kD vicilin-like protein as BW major allergens.
Objective: To investigate clinical usefulness of sIgE measurements against recombinant 16- and 19-kD BW allergens compared to the skin prick test and measurement of specific IgE against whole buckwheat (WBW) extract.
Method: Eleven BW allergic patients, 11 asymptomatic sensitizers to BW and 16 non-atopic subjects were enrolled. We performed the skin prick test, sIgE measurement against recombinant 16- and 19-kD BW allergens and WBW extract using a CAP system.
Result: In BW allergic patients, sIgE against WBW extract, r16- and r19-kD BW allergens were detected in 10, 5 and 9 patients, respectively. In asymptomatic sensitizers, sIgE against WBW extract, r16- and r19-kD BW allergens were
detected in 9, 1 and 2 patients respectively. In ROC analysis, the AUC value of sIgE level was higher in r19-kD allergen
than in r16-kD or WBW extract.
Conclusion: We suggest that CAP sIgE against r19-kD and r16-kD BW allergens can be a diagnostic marker to discriminate BW allergic patients from asymptomatic sensitizers in a clinical setting.
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KEYWORD
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Buckwheat, Allergy, Recombinant, Allergen, Specific IgE measurement
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