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KMID : 0892920200290010070
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Experimental Neurobiology
2020 Volume.29 No. 1 p.70 ~ p.79
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Expression of Cellular Receptors in the Ischemic Hemisphere of Mice with Increased Glucose Uptake
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Lee Jin-Soo
Hong Ji-Man
Yoon Bok-Seon
Son Keoung-Sun
Lee Kyung-Eon
Im Doo-Soon
Park Bok-Nam
An Young-Sil
Hwang Dong-Hoon
Park Chan-Bae
Kim Byung-Gon
Joe Eun-Hye
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Abstract
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Many previous studies have shown reduced glucose uptake in the ischemic brain. In contrast, in a permanent unilateral common carotid artery occlusion (UCCAO) mouse model, our pilot experiments using 18F-fluorodeoxyglucose positron emission tomography (FDG PET) revealed that a subset of mice exhibited conspicuously high uptake of glucose in the ipsilateral hemisphere at 1 week post-occlusion (asymmetric group), whereas other mice showed symmetric uptake in both hemispheres (symmetric group). Thus, we aimed to understand the discrepancy between the two groups. Cerebral blood flow and histological/metabolic changes were analyzed using laser Doppler flowmetry and immunohistochemistry/Western blotting, respectively. Contrary to the increased glucose uptake observed in the ischemic cerebral hemisphere on FDG PET (p<0.001), cerebral blood flow tended to be lower in the asymmetric group than in the symmetric group (right to left ratio [%], 36.4±21.8 vs. 58.0±24.8, p=0.059). Neuronal death was observed only in the ischemic hemisphere of the asymmetric group. In contrast, astrocytes were more activated in the asymmetric group than in the symmetric group (p<0.05). Glucose transporter-1, and monocarboxylate transporter-1 were also upregulated in the asymmetric group, compared with the symmetric group (p<0.05, respectively). These results suggest that the increased FDG uptake was associated with relatively severe ischemia, and glucose transporter-1 upregulation and astrocyte activation. Glucose metabolism may thus be a compensatory mechanism in the moderately severe ischemic brain.
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KEYWORD
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Brain ischemia, 4-fluoro-4-deoxyglucose, Positron-emission tomography, Glucose transporter type 1, Astrocytes
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