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Rat embryos were cultured out of the mother from the head-fold stages, 9.5 days to 11.5 days during which they start to develop the brain, eyes, ears and cardiovascular system etc. We principally did the basic experiment in order to establish the best condition of the rat whole embryo culture in our laboratory. The temperature in the culture system was maintained 370.2 for 48 hrs. The culture was carried out in humidified atmosphere of the air, intially, the bottles were equilibrated with 5% ,5% , and 90% gas mixture. After 22 or 24 and 29 or 30 hrs the cultures were reequilibrated with 20% , 5% ,75% and 40% , 5% , 55%respectively. Various types of sera were tested and for the purpose of minimizing the quantity of rat serum, artificial medium was also tried and it was determined that rat serum supported normal growth over a period of 48 hrs, based on total growth analysis and structural comparisons with in vivo specimens.
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