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KMID : 0893319960110030053
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Journal of Environmental Toxicology
1996 Volume.11 No. 3 p.53 ~ p.57
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Effects of Hydroxybrazilin on Glutathione Depletion Induced by and Menadione in Cultured Rat Hepatocytes
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Chang Eun-Sook
So Dhong-Su
Kim Jin-Hyoung
Jeon Sun-Duck
Moon Chang-Kiu
Park Kwang-Sik
Kim Sung-Gon
Khil Lee-Yong
Chang Tong-Shin
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Abstract
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In this study we investigated the effect of hydroxybrazilin on glutathione depletion induced by BrCCl$_3$ and menadione in cultured hepatocytes to understand the cellular mechanisms of hepatoprotective effect of hydroxybrazilin. Hydroxybrazilin alone had no effect on total glutathione level and the ratio of reduced glutathione/total glutathione (GSH/(GSSG+GSH)). BrCCl$_3$ dramatically decreased total glutathione level and hydroxybrazilin significantly prevented glutathion depletion by BrCCl$_3$. The ratio of GSH/(GSSG+ GSH) was also decreased by BrCCl$_3$ and recovered by hydroxybrazilin treatment. Menadione decreased total glutathione level and the ratio of GSH/(GSSG+GSH) but hydroxybrazilin showed no significant effects on menadione-induced glutathione depletion. These data suggest that hydroxybrazilin might prevent the hepatotoxicity induced by chemicalderived radicals but not the toxicity linked with oxidative stress.
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