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KMID : 0893320000150040147
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Journal of Environmental Toxicology
2000 Volume.15 No. 4 p.147 ~ p.155
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Protective Effect of Natural Medicinal Plants against Oxidative Damage Induced by Reactive Oxygen Species
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Lee Si-Eun
Ju Eun-Mi
Kim Jeong-Hee
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Abstract
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In order to evaluate anti -oxidant activities and protective effect against oxidatve damage, DPPH radical scavenging activity and lipid peroxidation inhibitory activity were measured among methanol extracts prepared from natural medicinal plants. Fourteen natural medicinal plants which were reported to have anti -oxidative or anti-inflammatory effects were selected based on our previous report. In addition to the total methanol extracts, n-hexane, dichloromethane, ethylacetate, n-butanol and water fractions were prepared from each total extract. DPPH radical scavenging assay was performed against 14 total extracts and all samples showed dose-dependent activities in various extent. Among those, 6 samples, methanol extracts of Euryale ferox, paeonia suffruticosa, Areca catechu var. dulcissima, Cinnamomun cassia, Alpinia katsumadai and Betula platyphlla var. japonica showed IC50/ value lower than 6.0 /ml. The highest DPPH radical scavenging activity was found in ethylacetate fraction of paeonia suffruticosa with IC50/ value of 1.1 /ml. Analysis of lipid peroxidation inhibitory activity on hydrogen peroxide-induced oxidative damage in Chinese hamster lung fibroblast (V79-4) cells revealed that the highest inhibitory effect was observed in methanol extract of Betula platyhpylla var. japonica. Lipid peroxidation inhibitory activity was observed as a dose-dependent manner in all samples used in this study. Among fraction samples, ethylacetate fraction of Alpinia katsumadai had the strongest inhibitory activity with IC50/ value of 0.9 /ml.
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KEYWORD
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Natural medicinal plants, DPPH radical scavenging activity, Lipid peroxidation inhibitory activity
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