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KMID : 0893320010160040197
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Journal of Environmental Toxicology
2001 Volume.16 No. 4 p.197 ~ p.204
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Studies on the Endocrine Disruption in Wildlife Fish
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Koog Ja-min
Ryu Ji-Sung
Jeong Gyu-Hyeok
Lee Chul-Woo
Park Eung-Roh
Park Kwang-Sik
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Abstract
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Endocrine disruption in crucian carp (Carassius auratus) living in the branch of Han River were examined. Vitellogenin level in plasma was measured using ELISA system and aromatase mRNA level in brain was observed using RT-PCR technique. In all female fish, vitellogenin levels were in the range of 20∼40 /ml and aromatase mRNA expression could be detected on the agarose gel after RT-PCR. However, in case of males, vitellogenin level was elevated in only one fish, while vitellogenin was hardly detected in others. Aromatase was expressed in all males although the levels were relatively lower than the level in female fish. Testis-ova and any other histological changes of reproductive organ were not shown in both sexes.
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KEYWORD
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Vitellogenin, Aromatase, RT-PCR, Crucian carp
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