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KMID : 0893320020170030265
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Journal of Environmental Toxicology
2002 Volume.17 No. 3 p.265 ~ p.272
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Detection for Multiresidue of the Organophosphorus and Carbamate Pesticides by Enzyme-Inhibition Method
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Kim Jung-Ho
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Abstract
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This study was carried out with the detection for multiresidue of the organophosphorus pesticides such as malathion, parathion. diazinon, and carbamate pesticide such as carbaryl, by enzyme-inhibition method. The acetylcholinesterase (AChE) and cholinesterase (ChE) activities in chicken brain determined by the Ellman’s method were 166.6 and 5.8 mol/min/g protein, and in chicken plasma were 23.1 and 8.3 mol/min/g protein, respectively. The optimum pH of AChE and ChE was 8.2 and 7.8, respectively. The Km of AChE and ChE was 0.034 and 0.045 mM, respectively. I50/ for AChE and ChE by some organophosphorus was 55.82 and 99.42 mg/L of malathion, 31.16 and 29.13 mg/L of parathion, and 17.89 and 19.62 mg/L of diazinon, respectively. I50/ for AChE and ChE by carbaryl of carbamate was 0.10 and 0.05 mg/L, respectively. The 0.07 mg/L of drinking water advisory level for carbaryl could be detected with I50/ of AChE and ChE. Enzyme-Inhibition (EI) method with AChE and ChE was used the multiresidue method to detect the 1 mg/L of the carbamate pesticides.
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KEYWORD
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Multiresidue method, Enzyme inhibition, Bioindicators, Acetylcholinesterase, Cholinesterase, Organophosphorus pesticides, Carbamate pesticides
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