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KMID : 0893320030180040295
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Journal of Environmental Toxicology
2003 Volume.18 No. 4 p.295 ~ p.303
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Changes in Cellular Viability and Peroxidase Activities of Green Algae Selenastrum capricornutum (Chlorophyceae) to Cadmium
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Choi Eun-Joo
Lee Sang-Goo
Lee Seung-Jin
Moon Sung-Kyung
Park Yong-Seok
Rhie Ki-Tae
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Abstract
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Physiological cellular activities responses to cadmium (Cd) exposure in green algae with several reductases activities and viability of the cell were examined. The cell division of green algae, Selenastrum capricornutum treated with 5ppm was significantly decreased than that of normal algae. The mean cell number of normal algal culture was as twice much as than that of algae at 6 days after Cd treatment. The cellular viability of algae was analysed by flow-cytometry with fluorescent dye after esterase reaction on cell membrane. The 85.35% of cellular viability of normal culture was decreased to 34.35% when algae was treated with 5 ppm of Cd at 6 days after treatment. It was considered that those method of flow-cytometry is useful tool for toxicity test on micro-organisms in the respect of identifying cellular viability. Also, the activities of both glutathione peroxidase (GPX) and ascorbate peroxidase (APX), which are indirectly react against oxidative stress through reduction of glutathione by Cd were significantly increased with 25%. It is considered that both GPX and APX are involved in the metabolic pathway of Cd -detoxification with similar portion in Selenasturm capricornutum.
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KEYWORD
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cadmium toxicity, green algae, cellular vitality, peroxidase activities
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