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KMID : 0893320060210030291
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Journal of Environmental Toxicology
2006 Volume.21 No. 3 p.291 ~ p.299
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Early Exposure to Anti-androgen Compounds Induces the Delay in the Testis Development in Immature Male Rat
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Hong Jin-Tae
Han Soon-Young
Moon Hyun-Ju
Kang Tae-Seok
Kang Il-Hyun
Kim Tae-Sung
Kim Seung-Hee
Kwon Ki-Sung
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Abstract
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The experiments investigated whether early exposure to testosterone propionate (TP) during prepuberty alters testis development in Sprague-Dawley male rats. We performed Hershberger assay using the stimulated weanling male rats by OECD protocols, cDNA microarray, and Western blot. TP was subcutaneously injected to uncastrated Sprague-Dawley male rat of 22 days old for 10 consecutive days at doses of 0.4, 0.8, 1.0, 1.2, 1.6 mg/kg per day. At necropsy, the following tissues were removed and weighed: combined testes, epididymides (Epi), Cowper’s glands (COW), levator am, and bulbocavernosus muscles (LABC), seminal vesicles, together with coagulating gland (SV) and ventral prostate (VP). We found that TP increased the weights of Epi, VP, SV, COW, and LABC, while testis was decreased in a dose-dependent manner. In cDNA microarray analysis of testis, there were significant reductions in the expression of cytochrome P450 11A (CYP11A), the rate-limiting enzyme of steroidogenesis. Taken together these results, TP exposure before puberty in male rats may produce the delay in testis development by inhibiting the CYP11A gene expression.
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KEYWORD
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OECD validation, Hershberger assay, TP, immature male rat
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