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KMID : 0893320070220040357
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Journal of Environmental Toxicology
2007 Volume.22 No. 4 p.357 ~ p.366
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Increased Genotoxicity of N’-methyl-N’-nitroguanidine by Oxidative Stress
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Kang Jin-Seok
Jung Ki-Kyung
Suh Soo-Kyung
Kim Ju-Hwan
Lee Hwa Ok
Jung Hai-Kwan
Kim Seung-Hee
Park Sue-Nie
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Abstract
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To investigate the possible enhancement of genotoxicity in stress environment, we examined the of effect of genotoxic material in oxidative stress-induced condition using human tell line. Human lymphoblast cell line, TK6 was treated with hydrogen peroxide () for induction of oxidative stress, and treated with N’-methyl-N’-nitroguanidine (MNNG), af a genetoxic material. We carried out MTS assay to set treatment doses. TK6 was treated with at 6.75 (low dote) or (high dose) for 2 h, and treated with MNNG af 0.117 (low dose), 0.234 (middle dose), (high dose) for 2 h. As results, a treatment of MNNG induced DNA dam age as dose dependently. And TK6 treated with at low as well as high dose followed by MNNG treatment showed higher DNA damage compared to MNNG alone treated groups. Malondialdehyde, as a marker of lipid peroxidation was increased in and MNNG treated groups. Real-time RT-PCR analyses for expression of several antioxidative enzymes showed that catalase mRNA and glutathione peroxidase 1 mRNA expression were decreased in and MNNG treated groups. Taken together, we conclude that genotoxicity induced by MNNG is enhanced in a condition of oxidative stress induced by and it suggests that it should be associated with induction of lipid peroxidation and decrease of antioxidant enzymes.
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KEYWORD
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genotoxicity, oxidative stress, hydrogen peroxide (), N’-methyl-N’-nitroguanidine (MNNG), TK6 cell line
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