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KMID : 0893420190200010079
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Journal of Veterinary Science
2019 Volume.20 No. 1 p.79 ~ p.86
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Iodixanol supplementation during sperm cryopreservation improves protamine level and reduces reactive oxygen species of canine sperm
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Abdillah Dimas A.
Setyawan Erif M. N.
Oh Hyun-Ju
Ra Ki-Hae
Lee Seok-Hee
Kim Min-Jung
Lee Byeong-Chun
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Abstract
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The objective of this study was to analyze the protective effects of iodixanol on dog spermatozoa during cryopreservation. The optimal concentration of iodixanol, 1.5%, was determined using fresh spermatozoa and was applied in the following experiments. The 1.5% iodixanol group showed significantly increased sperm motility from that in the control (p < 0.05). Lower mitochondrial reactive oxygen species (ROS) modulator (ROMO1) and pro-apoptotic gene (BAX) expressions, together with higher expressions of protamine-2 (PRM2), protamine-3 (PRM3), anti-apoptotic B-cell lymphoma-2 (BCL2), and sperm acrosome associated-3 (SPACA3) genes were detected in the iodixanol-treated group. In addition, decreased protamine deficiency and cryocapacitation were observed in the treatment group. Our results show that supplementation with 1.5% iodixanol is ideal for reducing production of ROS and preventing detrimental effects during the canine sperm cryopreservation process, effects manifested as increased motility and reduced cryocapacitation in frozen-thawed spermatozoa.
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KEYWORD
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Dogs, Sperm, Cryopreservation, Reactive oxygen species, Protamine
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