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KMID : 0893420190200010091
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Journal of Veterinary Science
2019 Volume.20 No. 1 p.91 ~ p.94
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Genetic identification of astroviruses in wild boars
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Vilcek Stefan
Salamunova Slavomira
Jackova Anna
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Abstract
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Astroviruses are widely detected in pigs but their detection in wild boars is rather sporadic. In this study, astroviruses were detected in organ homogenates of wild boars by applying nested reverse transcriptase polymerase chain reaction, and the typing was carried out by phylogenetic analysis. Overall, 30/200 (15.0%) homogenates were positive for astroviruses. Genetic typing revealed that of 13 amplicons analyzed, 8 were typed as porcine astrovirus lineage 2 (PAstV-2), 2 as lineage 4 (PAstV-4), 2 identical sequences were grouped with chicken astrovirus, and 1 sequence belonged to a bat astrovirus lineage. This first identification of chicken and bat astroviruses in wild boars indicates interspecies transmission.
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KEYWORD
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Wild boar, Porcine astrovirus, Chicken astrovirus, Bat astrovirus, Phylogenetic analysis
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