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KMID : 0893420200210010004
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Journal of Veterinary Science
2020 Volume.21 No. 1 p.4 ~ p.4
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Rapid detection of deformed wing virus in honeybee using ultra-rapid qPCR and a DNA-chip
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Kim Jung-Min
Lim Su-Jin
Kim So-Min
Kim Moon-Jung
Kim Byoung-Hee
Tai Truong A.
Kim Seon-Mi
Yoon Byoung-Su
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Abstract
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Fast and accurate detection of viral RNA pathogens is important in apiculture. A polymerase chain reaction (PCR)-based detection method has been developed, which is simple, specific, and sensitive. In this study, we rapidly (in 1 min) synthesized cDNA from the RNA of deformed wing virus (DWV)-infected bees (Apis mellifera), and then, within 10 min, amplified the target cDNA by ultra-rapid qPCR. The PCR products were hybridized to a DNA-chip for confirmation of target gene specificity. The results of this study suggest that our method might be a useful tool for detecting DWV, as well as for the diagnosis of RNA virus-mediated diseases on-site.
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KEYWORD
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Ultra-rapid reverse transcription-qPCR, DNA-chip, deformed wing virus, field detection
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