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KMID : 0894520030070010049
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Development & Reproduction
2003 Volume.7 No. 1 p.49 ~ p.56
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Effects of GnRH Agonist Administered to Mouse on Apoptosis in Ovary and Production of Estradiol and Progesterone
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홍순정/Hong SJ
양현원/김미란/이치형/황경주/권혁찬/윤용달/Yang HW/Kim MR/Lee CH/Hwang KJ/Kwon HC/Yoon YD
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Abstract
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There have been reports that administrated high-dose gonadotropin-releasing hormone-agonist(GnRH-Ag) suppresses endogenous gonadotropin production and inhibits function of ovary. In human IVF-ET program, however, GnRH-Ag is employed in large amounts during superovulation induction resulting to luteal phase defects which must be supported with progesterone. To elucidate the reason of luteal phase defects by GnRH-Ag, the aim of this study was to investigate the apoptosis changes in the ovary and the hormonal changes in the serum after GnRH-Ag and PMSG administration in adult mice in a method similar to human superovualtion induction. GnRH-Ag(10μg) or saline was injected every 12h beginning 48h prior to PMSG injection until 48h after PMSG injection when blood sampling and ovary collection was performed. In results, the ovary weight in the GnRH-Ag only injection group was significantly lower when compared with the other two groups, PMSG only or PMSG + GnRH-Ag injection. The ratio of preantral follicles in the ovary are increased in the GnRH-Ag only group, while the ratio of antral follicles are decreased and the corpus luteum ratio is increased in the PMSG + GnRH-Ag group. The proportion of all follicles showing apoptosis in the GnRH-Ag only injection group was seen to be more than twice the proportion seen in the PMSG only injection group, and such increased apoptosis is decreased after addition of PMSG. The serum levels of both estradiol and progesterone were significantly lower in the GnRH-Ag only group compared to those in the other two groups. When the administration of GnRH-Ag were followed by PMSG injection, however, estradiol concentration was completely recovered compared to the serum level of PMSG group, but not progesterone level. In conclusion the use of GnRH-Ag in human IVF-ET program may induce the apoptosis and the suppression of hormone production by ovary leading to luteal phase defects, thus adequate progesterone support seems to be necessary against them.
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KEYWORD
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Apoptosis, Luteal phase defects, GnRH agonist, Ovary, Progesterone
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