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KMID : 0900919840080010016
Korean journal of Animal Reproduction
1984 Volume.8 No. 1 p.16 ~ p.21
Comparison of Non-surgical and Surgical Recovery of Fertilized Eggs in Superovulated Rabbits
Shim Keum-Seob


Abstract
This experiment was carried out to compare with the nonsurgical and surgical recovery of fertilized eggs in super-ovulated rabbits. Sixty-four eggs recovered were transferred to twelve synchronized, pseudopregnant rabbits to test the viability of the eggs by surgical transfer. Each group(I, II, III) received a single subcutaneous injection of 5mg PGF2{alpha}/kg B.W. at 24(Group I), 48(Group II) and 72 hours (Group III) after mating, respectivity. After the administration of PGF2{alpha}, vaginal washings were conducted at 3, 6, 9, 12 and 24 hrs, and frequency of vaginal washing was 5 times for the each group (I, II, III). In Group (IV, V, ¥µ), the rabbits were killed to recover the fertilized eggs from the genital tract at 24(Group ¥µ), 48(Group V) and 72 hours (Group ¥µ) after mating, respectively. The results obtained were as follows: 1. Of the total eggs, 69.3%, 73.4% and 66.9% were recovered for Group I, II and III, respectively from the vagina within 6 hrs after PGF2{alpha} injection and particularly for Group III. 2. The rates of egg recovery versus the number of corpora lutea were 55 (51.6-60%), 35.8 (24-52.6%), 33.4 (25-47%) and 72 (70.7-73.0)%, 60.3 (50-71.4)%, 449(44.4-45.5)% in Group I, II, III and Group IV, V, ¥µ, respectively. 3. Most of eggs recovered were one-cell stage in Group Iand Group IV. More than one half of the eggs recovered in Group II and V were over eight-cell stage, and most of the eggs were so in Group III and ¥µ. 4. When sixty-four eggs recovered between 24 to 72 hours after mating were transferred to pseudopregnant rabbits. Three recipients were pregnant, and the rate of pregnancy was 25%.
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