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KMID : 0900919850090020148
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Korean journal of Animal Reproduction
1985 Volume.9 No. 2 p.148 ~ p.152
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Indirect Assessment of Sperm Capacitation Using Zona-free Hamster Eggs in the Goat I. Penetration into Zona-free Hamster Eggs by Goat Spermatozoa Preincubated in the Uteri Isolated from Hamsters and Rats
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Song H.-B.
Akira Iritani
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Abstract
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When goat spermatozoa were preincubated for 4-6 h and 6 h in the uteri isolated from hamster and rat, and for 6 h in the hamster uterus in situ, they developed the ability to penetrate zona-free hamster eggs in vitro. Zona-free hamster eggs were not penetrated after insemination with goat spermatozoa preincubated in the isolated hamster uterus 4 h before and 2 h after expected time of ovulation, respectively. Zona-free hamster eggs were not penetrated after insemination with goat spermatozoa preincubated for 4 h in the isolated hamster uterus, but 10 and 18% of eggs were penetrated by spermatozoa preincubated for 5 and 6 h in the isolated uterus.
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