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KMID : 0900919860100010109
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Korean journal of Animal Reproduction
1986 Volume.10 No. 1 p.109 ~ p.120
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Studies on In Vitro Capacitation by Lysolecithin and In Vitro Fertilizing Ability of Ejaculated Rabbit Sperm
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Kim C.-K.
Im K.-S.
Zheng, X.
R,H. Foote
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Abstract
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This study was conducted to define the effect of addition of lysolecithin (LC) and 20% v/v rabbit serum to sperm preincubation medium on the induction of acrosome reaction (AR) an fertilizing ability in vitro of LG-added sperm. Ejaculated rabbit sperm from New Zealand White buck was washed once by centrifugation, then preincubated for 2 or 4 hrs in a chemically defined medium (DM), DM plus 20% rabbit serum or BSA-free DM plus 20% rabbit serum at 37^{circ}C water bath or CO2 incubator. At the end of preincubation LC was added to the preincubated sperm, which was stained at 0.5 to 4 hr later and examined for AR and sperm motility. For in vitro fertilization, gametes were coincubated in DM up to 24 hrs and thereafter fertilized embryos were incubated in BSM -II up to 48 hrs. Addition of LC to 4-hr preincubated sperm was more effective for the AR and sperm motility than that to 2-hr preincubated sperm and optimal concentration of LC for AR was about 80{mu}g/ml. A significant increase in AR occured from 20 to 30 min. after addition of 80 to 100{mu}g/ml in 4-hr preincubated sperm. BSA-free DM plus 20% rabbit serum showed a higher AR and sperm motility than those of DM plus 20% rabbit serum in LC-added sperm after 4-hr preincubation. The incidence of AR after 4-hr preincubation and at 30 min after 60{mu}g/ml LC addition varied greatly among individual bucks. Sixty {mu}g/ml LC-added sperm showed a slight high cleavage rate over control levels, but 100{mu}g/ml LC-added sperm showed lower cleavage rate rather than 60{mu}g/ml LC. It is concluded that optimal concentration of LC for high AR induction and sperm motility in 4-hr preincubated sperm was about 80{mu}g/ml, but 60{mu}g/ml level was more useful for in vitro fertilization.
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KEYWORD
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