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KMID : 0900919880120010051
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Korean journal of Animal Reproduction
1988 Volume.12 No. 1 p.51 ~ p.62
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Production of Monoclonal Antibody to Human Chorionic Gonadotropin(hCG) : Purification and Properties of a Monoclonal Antibody, and Immunochemiluminometric assay(ICMA) for the Assay of hCG
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Lee Byung-Cheol
Lee Yong-Hwan
Kim Jong-Bae
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Abstract
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Spleen cells of mouse immunized with hCG were fused with myeloma cell (SP 2/0 Ag 14) to produce monoclonal antibody against hCG. Several clones of hybridoma secreting monoclonal antibody were established and antibodies were characterized in terms of titer, subisotyping and sensitivity in immunoassay. Several methods, for the purification of anti￢bodies, based on gel-filtration, DEAE-ion exchange chromatography and affinity chromatography. were applied and compared each other by the result of SDS-PAGE. Two-site immunochemiluminometric assay (ICMA) involving the use of an excess concentration of a specific monoclonal antibody passively adsorbed onto the walls of plastic tubes and a chemiluminescence labelled antibody conjugate were de￢veloped for the determination of hCG as a preliminary study
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