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KMID : 0900919890130010049
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Korean journal of Animal Reproduction
1989 Volume.13 No. 1 p.49 ~ p.55
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Cytogenetic Studies of Polyploidy Manipulation
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Abstract
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The Results of colchicine and pretreatment time have shown that the cells of Coho Salmon(Oncorhynchus Kisutch) were effectively injected 6 hours prior to the harvest at a final concentration of 10mutextrm{g}/g body weight. The cell-density from 8,000 cells/㎣ (=8{times}106 ml) was found as ideal. The best effect of hypotonic solution in proportion to the cell is 20 : 1 and 50 minutes. The model number of diploid chromosome in this species was 2n=60. The karyotype analysis proved that the diploid complement consisted of 19 pairs of metacentric-, 4 pairs of submetacentric- and 7 paris of acrocentric-chromosomes. The diagrammatic representation proved that the diploid complement consisted of 7 pairs of metacentric-, 2 pairs of submetacentric- and 1 pair of acrocentric-chromosomes.
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