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KMID : 0900919900140010031
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Korean journal of Animal Reproduction
1990 Volume.14 No. 1 p.31 ~ p.35
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Assessing the Viability of Rat Oocyte by Use of Eosin B
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Kim Myung-Cheol
Yun Hyo-In
Kim Moo-Kang
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Abstract
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The viability and developmental potential of rat oocyte was assessed by the use of eosin B. Dye was prepared as a stock solution to contain 1.0 mg eosin B per ml of Brinster’s Medium for Ovum Culture plus glucose (BMOC) -2 medium. Three dye concentrations were selected for the Oocytes from rat ovarian follicles were collected and assessed under various conditions of heat oocyte studies by dilution of the stock solution with BMOC: 0.12mM, 0.60mM and 1.20mM. treatment and preservation duration by dye exc￢lusion assay using eosin B. It is suggested that live oocyte excludes eosin B whereas dead oocyte does not.
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