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KMID : 0900919900140010043
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Korean journal of Animal Reproduction
1990 Volume.14 No. 1 p.43 ~ p.49
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Factors Affecting the Survival of Rabbit Embryos Cryopreserved by Vitrification
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Yang B.-S.
Oh S.-J.
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Abstract
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To improve the freezing techniques of animal embryos using vitrification solution as a cryoprotectant rabbit embryos, by cell stages, dehydration temperature and dehydration temperature and dehydratin time, were frozen-thawed and cultured. Following are the main results obtained. 1. The damage rate of zona pellucida after thawing was higher(13.6%) when the cell stage of embryos was less than 4 cells than when the cell stage was 8~16 cell or morula. The damage rate was higher when the dehydration temperature was 4^{circ}C than -30^{circ}C or -50~-80^{circ}C. The zona pellucida was damaged more when dehydrated for 5 min than when dehydrated for 10~15 min. 2. After being cultured for 72 hours, 5.3% of 4 cell(or less) embryos were developed to morula, while 86.4% of morula embryos were developed further. 3. More percentage of embryos(73.2%) was developed when dehydrated at -30^{circ}C than when dehydrated at 4^{circ}C at -50^{circ}C~-80^{circ}C$. 4. The hatching rate was higher when dehydrated for 5 min. When the embryos were dehydrated for 10~15 min and cultured for 24 hours, they were not even developed or development was not good in later stages
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