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KMID : 0900919960200020101
Korean journal of Animal Reproduction
1996 Volume.20 No. 2 p.101 ~ p.109
Comparision of Preservation of Liquid Boar Semen between Lactose-Egg Yolk and Bddot{u}tschwiler Diluents
Park Chang-Sik


Abstract
This study was done to find out the methods of long-term use of liquid boar semen in 100 ml plastic bottle for artificial insemination and to investigate differences between Lactose-Egg yolk and Biitschwiler diluents according to storage temperature, and effect of final glycerol concent ration in Lactose-Egg yolk diluent. Liquid boar semen diluted with Lactose-Egg yolk diluent showed significantly higher sperm motility (p<0.05) after 0.5 and 2h incubation at 37^{circ}C,than Butschwiler diluent at all storage length when it was preserved in the 5^{circ}C refrigerator. The NAR acrosome in Lactose-Egg yolk diluent a after 0.5 and 2h incubation at 37^{circ}C, respectively, during preservation periods was similar to that in Biitschwiler diluent. When liquid boar semen was preserved at 15^{circ}C, liquid boar semen in the Butschwiler diluent showed significantly higher percentages of sperm motility and NAR acrosome from third day to seventh than that in Lactose-Egg yolk diluent. In the effect of final glycerol concentration of liquid boar semen in the Lactose-Egg yolk diluent, the final glycerol concentration of 2% showed higer percentages of sperm motility and NAR acrosome than that of 0, 1, 3, and 5%. Farrowing rate, litter size and average pig weight at birth did not differ significantly between Lactose-Egg yolk and But schwiler diluents. As a result of this study, we found out that liquid boar semen can be stored for 6-7 days at 5^{circ}C in Lactose-Egg yolk diluent and at 15^{circ}C in Butschwiler diluent.
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