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KMID : 0900919970210010071
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Korean journal of Animal Reproduction
1997 Volume.21 No. 1 p.71 ~ p.78
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Parthenogenetic Activation and Development of Freshly Matured Bovine IVM Oocytes
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Cheong Hee-Tae
Park Choon-Keun
Yang Boo-Keun
Kim Choung-Ik
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Abstract
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This study was conducted to investigate the activation condition of freshly matured bovine IVM oocytes for use as a cytoplasmic recipient in nuclear transfer. Bovine oocytes matured in vitro for 22-24 h were treated with various activation conditions. In Experiment 1 in vitro matured oocytes were treated with electric stimuIus (ES; 2 pulses of 1.25 kV/cm for 70 {mu}{textrm{s}}ec, each pulse 1 sec apart), ethanol (ET; 7%, 5min) , Ca^2+-ionophore(A23187; 10mutextrm{g}/ml, 5min) and cycloheximide(CH; 10mutextrm{g}/ml, 6 h). Activation rates were similar in treatments with ES, ET and A23187(48.8~54.3%), however, significantly reduced with CH treatment(15.9%, P
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KEYWORD
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Parthenogenetic activation, Parthenogenetic development, Cycloheximide, Oocyte, Bovine
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