|
KMID : 0900919980220040395
|
|
Korean journal of Animal Reproduction
1998 Volume.22 No. 4 p.395 ~ p.403
|
|
|
In Vitro Development of Porcine Oocytes Following Intracytoplasmic Injection of Round Spermatid
|
|
Shin J.-S.
Kim K.-S.
Uhm S.-J.
Kim N.-H.
Lee H.-T.
Chung K.-S.
|
|
|
Abstract
|
|
|
|
In this study we determined fertilization processes and developmental ability of porcine oocytes following injection of round spermatid in the presence and absence of artificial activation. Electrical stimulation at 2 h before spermatid injection significantly increased the incidence of normal fertilization as compared to those following injection without stimulation or with stimulation immediately after injection. The incidences of two pronuclear formation and apposition were not different in oocytes between following intracytoplasmic spermatid and spermatid nucleus injection. Indirect immunocyto chemistry and laser scanning confocal microscopy study revealed that microtubuels were organized from the oocyte cortex following round spermatid injection, and this seemed to move both male and female pronuclei into the oocyte center. At 6 days following injection blastocoele formation was seen in the eggs following round spermatid (25%) and round spermatid nucleus injection (27%). However, none of oocytes developed to the blastocyst stage at 6 days following sham injection. The average cell numbers of blastocysts at 8 days following injection of spermatid and spermatid nucleus were 87 to 99. These results suggested that either round spermatid or it′s nudeus can be used to produce viable embryos by injection into unfertilized oocytes in the pig,
|
|
|
KEYWORD
|
|
|
Porcine, Oocyte, IVD, Round spermatid, Intracytoplasmic injection
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|