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KMID : 0900919990230030221
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Korean journal of Animal Reproduction
1999 Volume.23 No. 3 p.221 ~ p.227
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In Vitro Fertilization and Development of Bovine Immature Oocytes by Ultra- Rapid Cooling
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Ryu C.-S.
Kil K.-S.
Kim N.-H.
Ko D.-H.
Lee H.-T.
Chung K.-S.
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Abstract
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Successful cryopreservation of bovine immature oocytes can increase availably of oocytes for the in vitro fertilization or nuclear transfer. However, it was not reported successful development to the blastocyst stage following in vitro fertilization of cryopreserved bovine immature oocytes. The objective of this study was to determine the incidence of survival, meiotic maturation, fertilization and in vitro development of cryopreserved bovine immature by ultra rapid cooling methods. The oocytes were adversely affected by brief exposure to EFS40 solution in electron microscope grids and plunged directly into liquid nitrogen. After such ultra-rapid cooled immature oocytes were warmed, 78% of oocytes were matured to the metaphase II stage, 50% of oocytes were fertilized after insemination, and 5% of oocytes were developed to the blastocyst stage. Different sodium concentration of sodium ion in the freezing medium did not affect survival, maturation, fertilization and in vitro development of cryopreserved oocytes. These results suggested that immature bovine oocytes can be cryopreserved by ultra-rapid cooling methods.
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KEYWORD
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Cryopreservation, Bovine oocytes, In vitro fertilization, In vitro culture
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